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ecori spei fragment  (New England Biolabs)


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  • 99

    Structured Review

    New England Biolabs ecori spei fragment
    Ecori Spei Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14873 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecori+spei+fragment/EcoRI/pmc10561733__jciinsight___8___164816___s222-76-18-30
    Average 99 stars, based on 14873 article reviews
    ecori spei fragment - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Luciferase:

    Article Title: MicroRNA-1 protects the endothelium in acute lung injury
    Article Snippet: Luciferase assay: Angpt2 100-bp 3’UTR sequence (containing miR-1 binding site) was synthesized using oligos with EcoRI and SpeI sequences at the ends, followed by hybridization to make a double-stranded DNA insert. .. Luciferase vector pEZX-MT06 (GeneCopoeia, a gift from Maor Sauler, Yale University, New Haven) was opened by releasing the EcoRI/SpeI fragment and the Angpt2 insert was ligated using T4 DNA ligase (New England Biolab) followed by TOPO cloning (Invitrogen). ..

    Plasmid Preparation:

    Article Title: MicroRNA-1 protects the endothelium in acute lung injury
    Article Snippet: Luciferase assay: Angpt2 100-bp 3’UTR sequence (containing miR-1 binding site) was synthesized using oligos with EcoRI and SpeI sequences at the ends, followed by hybridization to make a double-stranded DNA insert. .. Luciferase vector pEZX-MT06 (GeneCopoeia, a gift from Maor Sauler, Yale University, New Haven) was opened by releasing the EcoRI/SpeI fragment and the Angpt2 insert was ligated using T4 DNA ligase (New England Biolab) followed by TOPO cloning (Invitrogen). ..

    Cloning:

    Article Title: MicroRNA-1 protects the endothelium in acute lung injury
    Article Snippet: Luciferase assay: Angpt2 100-bp 3’UTR sequence (containing miR-1 binding site) was synthesized using oligos with EcoRI and SpeI sequences at the ends, followed by hybridization to make a double-stranded DNA insert. .. Luciferase vector pEZX-MT06 (GeneCopoeia, a gift from Maor Sauler, Yale University, New Haven) was opened by releasing the EcoRI/SpeI fragment and the Angpt2 insert was ligated using T4 DNA ligase (New England Biolab) followed by TOPO cloning (Invitrogen). ..



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